Journal
GENE THERAPY
Volume 21, Issue 4, Pages 444-449Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2014.5
Keywords
lentivirus; CD4 T cells; shRNA; Vpx; HIV; AIDS
Categories
Funding
- UCSF/Robert John Sabo Trust
- NIH/NIAID [R21 AI102782, 1DP11036502, U19 AI096113]
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Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.
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