Journal
GENE THERAPY
Volume 21, Issue 1, Pages 37-43Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2013.55
Keywords
angiogenesis; brain; microRNA; microvessel; neurogenesis
Categories
Funding
- 973 Program of NBRP, China [2011CB504405]
- NSFC [30973097, 81200943]
- Shanghai medical association [SHNR-003]
- Shanghai healthy bureau [20124217]
- Science and Technology Commission of Shanghai Municipality [12ZR1418600]
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Angiogenesis and neurogenesis are crucial processes for brain tissue repair and remodeling after brain injury. Current study shows that microRNA-210 (miR-210) promotes vascular endothelial cell migration and tube formation under hypoxia in vitro. Whether miR-210 overexpression promotes focal angiogenesis and neurogenesis in the normal adult brain is unknown. Adult male C57BL/6 mice (n=54) underwent stereotactic injection of a lentiviral vector carrying miR-210 (LV-miR-210). Following 28 days of miR-210 gene transfer, endothelial cell and neural precursor cell proliferation, microvessel density and downstream angiogenic factor were genotyped. miR-210 was highly expressed in neurons, astrocytes and endothelial cells of the LV-miR-210-injected brain hemisphere. The endothelial cell proliferation and the number of newly formed microvessels were greatly increased in the LV-miR-210-treated mice compared with the controls (P<0.05). Neural progenitor cells in the subventricular zone were greatly increased compared with the controls (P<0.05). The data indicate that miR-210 is a key factor at the microRNA level in promoting angiogenesis and neurogenesis, which was associated with local increased vascular endothelial growth factor (VEGF) levels, suggesting that miR-210 may be a potential target for ischemic stroke therapy.
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