Journal
GENE THERAPY
Volume 19, Issue 1, Pages 94-100Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2011.77
Keywords
Minivector DNA; Minicircle; hydrodynamic shear; nebulized; aerosolized; lung delivery
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Funding
- Infection and Immunity Training Grant [T32 AI55413-05]
- University of Texas MD Anderson Cancer Center
- Clayton Foundation for Research
- National Institutes of Health [RO1A1054830]
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Supercoiled DNAs varying from 281 to 5302 bp were subjected to shear forces generated by aerosolization or sonication. DNA shearing strongly correlated with length. Typical sized plasmids (>= 3000 bp) degraded rapidly. DNAs 2000-3000 bp persisted similar to 10 min. Even in the absence of condensing agents, supercoiled DNA <1200 bp survived nebulization, and increased forces of sonication were necessary to shear it. Circular vectors were considerably more resistant to shearing than linear vectors of the same length. DNA supercoiling afforded additional protection. These results show the potential of shear-resistant Minivector DNAs to overcome one of the major challenges associated with gene therapy delivery. Gene Therapy (2012) 19, 94-100; doi:10.1038/gt.2011.77; published online 2 June 2011
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