Journal
GENE THERAPY
Volume 17, Issue 3, Pages 419-423Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2009.153
Keywords
electroporation; cardiovascular; VEGF; heart; nonviral
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Funding
- National Institutes of Health [R21 HL089017]
- University of South Florida College of Medicine
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Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly increased cardiac expression of VEGF compared with injection of plasmid alone. This is the first report showing successful intracardiac delivery, through in vivo EP, of a protein expressing plasmid in a large animal. Gene Therapy (2010) 17, 419-423; doi:10.1038/gt.2009.153; published online 3 December 2009
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