Journal
GENE THERAPY
Volume 17, Issue 3, Pages 305-314Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2009.146
Keywords
gene cell therapy; VEGF; angiogenic growth factors; cardiac repair; angiogenesis gene therapy; biopolymer scaffold
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [01/00090]
- Ministerio da Ciencia e Tecnologia
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Ministerio da Saude/Departamento Ciencia e Tecnologia [552324/20005-1, 10120104096700]
- FAPESP [04/06784-4, 03/02671-8, 03/02672-4]
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In this study, we analyzed whether transplantation of cardiac fibroblasts (CFs) expressing vascular endothelial growth factor (VEGF) mitigates cardiac dysfunction after myocardial infarction (MI) in rats. First, we observed that the transgene expression lasts longer (45 vs 7 days) when fibroblasts are used as vectors compared with myoblasts. In a preventive protocol, induction of cardiac neovascularization accompanied by reduction in myocardial scar area was observed when cell transplantation was performed 1 week before ischemia/reperfusion and the animals analyzed 3 weeks later. Finally, the therapeutic efficacy of this approach was tested injecting cells in a fibrin biopolymer, to increase cardiac retention, 24 h post-MI. After 4 weeks, an increase in neovascularization and a decrease in myocardial collagen were observed only in rats that received cells expressing VEGF. Basal indirect or direct hemodynamic measurements showed no differences among the groups whereas under pharmacological stress, only the group that received cells expressing VEGF showed a significant reduction in end-diastolic pressure and improvement in stroke volume and cardiac work. These results indicate that transplantation of CFs expressing VEGF using fibrin biopolymer induces neovascularization and attenuates left ventricle fibrosis and cardiac dysfunction in ischemic heart. Gene Therapy (2010) 17, 305-314; doi:10.1038/gt.2009.146; published online 10 December 2009
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