Journal
GENE EXPRESSION PATTERNS
Volume 12, Issue 5-6, Pages 213-218Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gep.2012.03.005
Keywords
Setdb1/Eset; Mouse embryo; Histone methylation; Blastocyst outgrowth; Embryonic stem cell
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Funding
- National Research Foundation of Korea [2011-0030769, 2010-0021001]
- Next-generation BioGreen21 Grant [PJ008002]
- National Research Foundation of Korea [2011-0030769, 2010-0021001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expression was markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4. (c) 2012 Elsevier B.V. All rights reserved.
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