Journal
GENE EXPRESSION PATTERNS
Volume 8, Issue 6, Pages 382-388Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gep.2008.05.002
Keywords
GAP-43; axon growth; gene expression; neuronal development; regeneration; adult neurogenesis; transgenic zebrafish; fugu; CNS; PNS; GFP; reporter gene
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Funding
- NICHD NIH HHS [P01HD039948-010004] Funding Source: Medline
- NINDS NIH HHS [R03 NS055154-01, R03 NS055154-02, R03 NS055154] Funding Source: Medline
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Unlike mammals, fish have the capacity for functional adult CNS regeneration, which is due, in part, to their ability to express axon growth-related genes in response to nerve injury. One such axon growth-associated gene is gap43, which is expressed during periods of developmental and regenerative axon growth, but is not expressed in CNS neurons that do not regenerate in adult mammals. We previously demonstrated that cis-regulatory elements of gap43 that are sufficient for developmental expression are not sufficient for regenerative expression in the zebrafish. Here we have identified a 3.6 kb genomic sequence from Fugu rubripes that can promote reporter gene expression in the nervous system during both development and regeneration in zebrafish. This compact sequence is advantageous for functional dissection of regions important for axon growth-associated gene expression during development and/or regeneration. In addition, this sequence will also be useful for targeting gene expression to neurons during periods of growth and plasticity. (C) 2008 Elsevier B.V. All rights reserved.
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