4.6 Article

Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco

Journal

GENE
Volume 531, Issue 1, Pages 53-63

Publisher

ELSEVIER
DOI: 10.1016/j.gene.2013.08.028

Keywords

Pelteobagrus fulvidraco; Gene cloning; Tissue expression; Lipid metabolism

Funding

  1. Fundamental Research Funds for the Central Universities [2013PY073]
  2. National Natural Science Foundation of China [31001101]
  3. Postgraduates Innovation Research Project of Huazhong Agricultural University [2009sc018]

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11 genes involved in lipid metabolism were cloned from liver of yellow catfish Pelteobagrus fulvidraco, including CPT 1A, CPT 1B, PPAR alpha, PPAR gamma, SREBP-1, G6PD, 6PGD, FAS, acetyl-CoAACCa, ACCb, and LPL Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of yellow catfish. mRNA of all eleven genes was present in liver, muscle, mesenteric adipose, ovary and heart, but at varying levels. The present study will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species. (C) 2013 Elsevier B.V. All rights reserved.

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