4.6 Article

First draft genome sequence of the Japanese eel, Anguilla japonica

Journal

GENE
Volume 511, Issue 2, Pages 195-201

Publisher

ELSEVIER
DOI: 10.1016/j.gene.2012.09.064

Keywords

Eel; Anguilla; Assembly pipeline; Illumina sequencing; De novo genome; Hox genes

Funding

  1. Centre National de la Recherche Scientifique
  2. L'Agence Nationale de la Recherche [08-BLAN-0173]
  3. Norwegian School of Veterinary Science
  4. Research Council of Norway [184851]
  5. Grants-in-Aid for Scientific Research [21228005] Funding Source: KAKEN

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The Japanese eel is a much appreciated research object and very important for Asian aquaculture; however, its genomic resources are still limited. We have used a streamlined bioinformatics pipeline for the de novo assembly of the genome sequence of the Japanese eel from raw Illumina sequence reads. The total assembled genome has a size of 1.15 Gbp, which is divided over 323,776 scaffolds with an N50 of 52,849 bp, a minimum scaffold size of 200 bp and a maximum scaffold size of 1.14 Mbp. Direct comparison of a representative set of scaffolds revealed that all the Hox genes and their intergenic distances are almost perfectly conserved between the European and the Japanese eel. The first draft genome sequence of an organism strongly catalyzes research progress in multiple fields. Therefore, the Japanese eel genome sequence will provide a rich resource of data for all scientists working on this important fish species. (C) 2012 Elsevier B.V. All rights reserved.

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