Journal
GENE
Volume 454, Issue 1-2, Pages 39-46Publisher
ELSEVIER
DOI: 10.1016/j.gene.2010.01.008
Keywords
Partial editing; Pentatricopeptide repeat protein; Mitochondrial ribosomal protein; Arabidopsis thaliana
Categories
Funding
- Ministry of Education, Science, Sports and Culture of Japan [15208001]
- Japan Society for the Promotion of Science for young scientists
- Grants-in-Aid for Scientific Research [15208001] Funding Source: KAKEN
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C-to-U RNA editing (i.e., alteration of a C in the genomic sequence to U in the transcript) has been confirmed widely in angiosperm organellar genomes. During the C-to-U RNA editing event, incomplete edited transcripts have been observed at many sites in the steady-state mRNA population (partial editing). Here, by using coexpression analysis and the surveillance of whole editing status on the mitochondrial genome, we have revealed that a pentatricopeptide repeat (PPR) protein classified into the P subfamily (PPR596) has site-specific influence on the efficiency of C-to-U RNA editing events at partial editing sites on the Arabidopsis thaliana mitochondrial genome. Previous works have revealed that PPR proteins classified into the PLS subfamily containing the E or E and DYW motif are involved in RNA editing as trans-factors; they are believed to recruit deaminase at editing sites. In contrast with the mutant analyses of PLS-subfamily PPR proteins, the editing efficiency at rps3eU1344SS was revealed to be significantly increased in ppr596 mutants. Our study implies P-subfamily PPR protein is involved in the control of the degree of partial editing. (C) 2010 Elsevier B.V. All rights reserved.
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