Journal
GENE
Volume 436, Issue 1-2, Pages 115-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2009.01.016
Keywords
F9; General transcription factor; Proteasome; Proteolysis; TFIIB; Ubiquitination; TLP
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Funding
- Nisshinbo Co. Ltd
- Grants-in-Aid for Scientific Research [20570123] Funding Source: KAKEN
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We found that the levels of all general transcription factors (GTFs) for RNA polymerase II decreased in F9 cells when the cells were subjected to a differentiation procedure. Different from other GTFs, decrease of TFIIB during the differentiation was suppressed by addition of a proteasome inhibitor, MG132. The half-life of TFIIB in the differentiated cells was remarkably reduced compared with that in the undifferentiated cells. Moreover, it was demonstrated that TFIIB is a poly-ubiquitinated protein. Results of this study suggest that components of the transcription machinery decreased in accordance with cell differentiation and that TFIIB is specifically and rapidly degraded by the ubiquitin-proteasome pathway. (C) 2009 Elsevier B.V. All rights reserved.
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