Journal
GASTROENTEROLOGY
Volume 145, Issue 5, Pages 966-+Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1053/j.gastro.2013.07.026
Keywords
Animal Model; Monkey; Hepatitis C Virus; Replication
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Funding
- Robin Chemers Neustein Postdoctoral Fellowship
- Jose Carreras/E.D. Thomas Chair for Cancer Research
- National Institutes of Health (NIH) [R01 HL098489, P51 RR00016, R01 DK095125, R00 AI077800, R56 AI091792]
- Black Family Stem Cell Institute at the Icahn School of Medicine at Mount Sinai and NIH [R01 DK087867]
- American Cancer Society Research Scholar Grant [RSG-12-176-01-MPC]
- Pew Charitable Funds
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The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.
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