Journal
GASTROENTEROLOGY
Volume 135, Issue 2, Pages 632-641Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1053/j.gastro.2008.05.026
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Funding
- Medical Research Council [G9900432, G0700167] Funding Source: Medline
- NIDDK NIH HHS [DK 37482, DK41301, DK 33850] Funding Source: Medline
- Medical Research Council [G0700167, G9900432] Funding Source: researchfish
- MRC [G0700167, G9900432] Funding Source: UKRI
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Background & Aims: Cholecystokinin (CCK) has been thought to act only indirectly on human pancreatic acinar cells via vagal nerve stimulation, rather than by direct CCK receptor activation as on rodent pancreatic acinar cells. We tested whether CCK (CCK-8 and human CCK-58) can act directly on human pancreatic acinar cells. Methods: Human acinar cells were freshly isolated from pancreatic transection line samples, loaded with Fluo4-AM or quinacrine, and examined for Ca2+, metabolic and secretory responses to CCK-8, human CCK-58,. or acetylcholine with confocal microscopy. Results: CCK-8 and human CCK-58 at physiologic concentrations (1-20 pmol/L) elicited rapid, robust, oscillatory increases of the cytosolic Ca2+ ion concentration, showing apical to basal progression, in acinar cells from 14 patients with unobstructed pancreata. The cytosolic Ca2+ ion concentration increases were followed by increases in mitochondrial. adenosine triphosphate production and secretion. CCK-elicited Ca2+ signals and exocytosis were not inhibited by atropine (1 mu mol/L) or tetrodotoxin (100 nmol/L), showing that CCK was unlikely to have acted via neurotransmitter release. CCK-elicited Ca2+ signals were inhibited reversibly by caffeine (5-20 mmol/L), indicating involvement of intracellular inositol trisphosphate receptor Ca2+ release channels. Acetylcholine (50 nmol/L) elicited similar Ca2+ signals. Conclusions: CCK at physiologic concentrations in the presence of atropine and tetrodotoxin elicits cytosolic Ca2+ signaling, activates mitochondrial function, and stimulates enzyme secretion in isolated human pancreatic acinar cells. We conclude that CCK acts directly on acinar cells in the human pancreas.
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