Journal
FUTURE ONCOLOGY
Volume 10, Issue 3, Pages 385-399Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/FON.13.209
Keywords
enzyme immunoassay; human breast cancer cell lines; JAA-F11 monoclonal antibody; monoclonal antibody; Thomsen-Friedenreich antigen
Categories
Funding
- NIH STTR
- CDMRP [W81XWH-04-1-0342]
- Oncologic Foundation of Buffalo
- UB STOR Product Development Fund
- Oishei Foundation
- Bruce Holm Catalyst Fund
- Mark Diamond Research Foundation of the State University of New York at Buffalo [SP-11-05]
- Mark Diamond Research Foundation
- Organization of American States
- National Cancer Institute [NIH 1 R41 CA176951-01]
- Bruce Holm Memorial Catalyst Fund
- NSF [1204209]
- NIAID [R15 AI 49210-01]
- Direct For Education and Human Resources
- Division Of Undergraduate Education [1204209] Funding Source: National Science Foundation
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Aim: The Thomsen-Friedenreich antigen (TF-Ag) is a disaccharide hidden on normal cells, but selectively exposed on the surface of breast, colon, prostate and bladder cancer cells. JAA-F11, a highly specific monoclonal antibody to TF-Ag, reduces metastasis and prolongs survival in a mouse model. In addition,I-124-JAA-F11 localizes 4T1 tumors in mice. These studies continue translation of JAA-F11 to human breast cancer. Materials & methods & results: Of the 41 human breast cancer cell lines tested, 78% were positive for reactivity with JAA-F11 by whole-cell enzyme immunoassay and positivity occurred unrelated to estrogen, progesterone or HER2 receptor status. JAA-F11 inhibited the growth rate of the human cancer cell lines tested. At 1 h, approximately 80% of JAA-F11 internalized in the three cell lines tested. I-124-JAA-F11 specifically imaged human triple-negative tumors in mice by microPET. Conclusion: The results highlight the potential that humanized JAA-F11 may have for immunotherapy and drug conjugate therapy in breast cancer patients.
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