Journal
FUNGAL GENETICS AND BIOLOGY
Volume 48, Issue 1, Pages 49-61Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2010.06.012
Keywords
Fungal metabolism; Non-ribosomal peptide synthetases; Polyketide synthases; Thiotemplate biosynthesis; Secondary metabolism; Proteomics; Mass spectrometry; Fourier-Transform mass spectrometry
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Funding
- National Institutes of Health [GM 067725]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM067725, P01GM077596] Funding Source: NIH RePORTER
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With many bioactive non-ribosomal peptides and polyketides produced in fungi, studies of their biosyntheses are an active area of research. Practical limitations of working with mega-dalton synthetases including cell lysis and protein extraction to recombinant gene and pathway expression has slowed understanding of many secondary metabolic processes relative to bacterial counterparts. Recent advances in accessing fungal biosynthetic machinery are beginning to change this. Here we describe the successes of some studies of thiotemplate biosynthesis in fungal systems, along with very recent advances in chemical tagging and mass spectrometric strategies to selectively study biosynthetic conveyer belts in isolation, and within a few years, in endogenous fungal proteomes. (C) 2010 Elsevier Inc. All rights reserved.
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