Journal
FUNGAL GENETICS AND BIOLOGY
Volume 47, Issue 9, Pages 753-760Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2010.06.002
Keywords
Botrytis cinerea; High-osmolarity glycerol (HOG) pathway; Vegetative differentiation; Virulence
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Funding
- National Key Basic Research and Development Program [2006CB101907]
- 863 Program [2007AA10Z422]
- National Science Foundation [30771430]
- Michigan Agricultural Experiment Station
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We present a characterization of bos5 from Botrytis cinerea, a gene that encodes a mitogen-activated protein kinase kinase (MAPKK), which is homologous to OS-5 of Neurospora crassa. The bos5 gene deletion mutant exhibited reduced vegetative growth and strongly impaired conidiation. The mutant also exhibited increased sensitivity to the dicarboximide fungicide iprodione and to osmotic stress mediated by NaCl or KCl. Western-blot analysis showed that the BcSAK1 protein, the putative downstream component of BOS5, was not phosphorylated in the mutant. Plant inoculation tests showed that the mutants were unable to infect cucumber leaves. All of these defects were restored by genetic complementation of the Delta bcos5-21 mutant with the wild-type bos5 gene. These results indicated that BOSS is involved in the regulation of vegetative differentiation, virulence, adaptation to iprodione and ionic stress in B. cinerea. (C) 2010 Elsevier Inc. All rights reserved.
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