Journal
FUNGAL GENETICS AND BIOLOGY
Volume 46, Issue 11, Pages 837-848Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2009.07.005
Keywords
Cryphonectria parasitica; Mitochondria; Plasmid-like element; Hypovirulence; rns gene; Group I intron; Group II intron
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Funding
- Natural Sciences and Engineering Research Council of Canada
- Conselho Nacional de Desenvolvimento Cientifico Tecnologico (CNPq/Brazil)
- USDA [95-37303-1785]
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The mt-rns gene of Cryphonectria parasitica is 9872 bp long and includes two group I and two group II introns. An analysis of intronic protein-encoding sequences revealed that LAGLIDADG ORFs, which usually are associated with group I introns, were transferred at least twice into group II introns. A plasmid-like mitochondrial element (plME) that appears in high amounts in previously mutagen-induced mit1 and mit2 hypovirulent mutants of the Ep155 standard virulent strain of C. parasitica was found to be derived from a short region of the mt-rns gene, including the exon 1 and most of the first intron. The plME is a 4.2-kb circular, multimeric DNA and an autonomously-replicating mtDNA fragment. Although sexual transmission experiments indicate that the plME does not directly cause hypovirulence, its emergence is one manifestation of the many complex molecular and genetic events that appear to underlie this phenotype. (C) 2009 Elsevier Inc. All rights reserved.
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