Does the growth rate hypothesis apply to aquatic hyphomycetes?

The growth rate hypothesis states that in many organisms or tissues, the specific growth rate mu correlates with RNA concentrations. Since RNA often accounts for much of the phosphorus content of cells, mu may also correlate positively with P concentrations and negatively with C:P and N:P ratios. We tested this hypothesis with broth cultures of five aquatic hyphomycete species. Samples were harvested on eight occasions after 3-56 d of incubation. Accumulation of biomass was fitted to a rectangular hyperbola, whose parameters were used to estimate mu. There were no consistent trends related to culture age or mu for C, N, P or ergosterol concentrations. RNA and DNA concentrations and RNA:DNA ratios were significantly and negatively correlated with culture age. Only RNA concentrations were positively and linearly correlated with mu. While RNA or DNA concentrations are unsuitable as indicators for total biomass, levels of fungal RNA combined with markers for fungal biomass may allow estimates of the extent to which the mycelia are metabolically active. (C) 2013 Elsevier Ltd and The British Mycological Society. All rights reserved.