Journal
FUNGAL ECOLOGY
Volume 4, Issue 1, Pages 115-119Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funeco.2010.09.005
Keywords
Active decomposing fungi; N-15-DNA stable isotope probing; Plant residue quality; Tropical soil
Funding
- National Foundation for Science, Technology, Innovation
- National Institute of Agricultural Research in Venezuela
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N-15-DNA stable isotope probing (N-15-DNA-SIP) combined with 18S rRNA gene-based community analysis was used to identify active fungi involved in decomposition of N-15-labeled maize and soybean litter in a tropical Vertisol. Phylogenetic analysis of N-15-labeled DNA subjected to 18S rRNA gene-based community fingerprinting showed that addition of maize residues promoted relatively slow-growing fungal decomposers (i.e. Penicillium spp., Aspergillus spp.), while addition of soybean residues promoted relatively fast-growing fungal decomposers (i.e. Fusarium spp., Mortierella spp.). Chaetomium spp. were dominant decomposers in both residue treatments. Therefore, we have clear evidence that specific members of the fungal community used N-15 derived from the two organic resources with contrasting biochemical quality for growth. Our study showed that N-15-DNA-SIP-based community analysis is a useful method to follow the fate of N from organic resources into the actively decomposing fungal community of soils. (C) 2010 Elsevier Ltd and The British Mycological Society. All rights reserved.
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