Journal
FUNGAL BIOLOGY
Volume 118, Issue 4, Pages 402-412Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2014.02.005
Keywords
Fungi; Gibberella fujikuroi; Mycotoxin; Rice pathogen; Bakanae disease
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [24580474]
- Research project for improving food safety and animal health
- Grants-in-Aid for Scientific Research [24580474] Funding Source: KAKEN
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PCR-RFLP based on the translation elongation factor 1 alpha (TEF) gene was developed to identify Fusarium fujikuroi in the Fusarium (Gibberella) fujikuroi species complex. Ninety-three strains, most of which were obtained from various sources in Japan, were identified as F. fujikuroi and their capability to produce fumonisin was investigated using an in vitro assay. Fumonisin production was detected in 50 strains isolated from maize, strawberry, wheat, and rice, whereas it was undetectable in 43 strains derived from rice seeds and rice seedlings carrying the bakanae disease, and from unknown sources. A single nucleotide polymorphism in the TEF gene (T618G) correlated with the ability to synthesize fumonisin. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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