Journal
FUNGAL BIOLOGY
Volume 118, Issue 7, Pages 591-600Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2013.11.011
Keywords
Aquaculture; ITS nrDNA; Oomycetes; Pathogen; RAPD
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Funding
- Ministerio de Economia y Competitividad [CGL2012-39357]
- European Commission through the EU Marie Curie ITN project SAPRO [238550]
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Some species of the genus Saprolegnia, such as Saprolegnia diclina and Saprolegnia ferax are responsible for devastating infections on salmonid eggs. Members of this group cause saprolegniasis, a disease resulting in considerable economic losses in aquaculture. Although both S. diclina and S. ferax have received much attention, the role of other Saprolegnia species in infecting fish eggs is less known. For this purpose, we have investigated the aetiology of chronic egg mortality events occurring in farmed brown trout, Salmo trutta. A total of 48 isolates were obtained from eggs with signs of infection as well as from water samples. A molecular analysis based on nrDNA internal transcribed spacer (ITS) operational taxonomic units indicated that the majority of the isolates correspond to Saprolegnia australis. All isolates of S. australis exhibited the same random amplified polymorphic DNA (RAPD) band patterns suggesting that a single strain is implicated in egg infections. The isolates followed Koch postulates using trout eggs and fry. Under standard concentrations of bronopol commonly used in farms, these isolates could grow, but not sporulate. However, both growth and sporulation were recovered when treatment was removed. This study shows that S. australis can infect and kill salmon eggs, and helps in defining oomycetes core pathogens. (C) 2013 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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