Journal
FUNCTIONAL & INTEGRATIVE GENOMICS
Volume 14, Issue 3, Pages 603-615Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-014-0385-0
Keywords
Salvia miltiorrhiza; Hairy roots; Tanshinone; Co-regulation; Elicitor
Categories
Funding
- National Natural Science Fund [31270007, 31201261, 30900110]
- Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China [131041]
- New Century Talent Project [NECT-13-0902]
- Shanghai Talent Development Fund
- Open Funding Project of the State Key Laboratory of Bioreactor Engineering, Shanghai Science and Technology Committee Project [10JC1412000, 09QH1401900]
- Shanghai Education Committee Fund [13ZZ104, 09ZZ138, J50401]
- Key Science and Technology Project of Yangtze River Delta [10140702018]
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Tanshinone is widely used for treatment of cardio-cerebrovascular diseases with increasing demand. Herein, key enzyme genes SmHMGR (3-hydroxy-3-methylglutaryl CoA reductase) and SmDXR (1-deoxy-d-xylulose 5-phosphate reductoisomerase) involved in the tanshinone biosynthetic pathway were introduced into Salvia miltiorrhiza (Sm) hairy roots to enhance tanshinone production. Over-expression of SmHMGR or SmDXR in hairy root lines can significantly enhance the yield of tanshinone. Transgenic hairy root lines co-expressing HMGR and DXR (HD lines) produced evidently higher levels of total tanshinone (TT) compared with the control and single gene transformed lines. The highest tanshinone production was observed in HD42 with the concentration of 3.25 mg g(-1) DW. Furthermore, the transgenic hairy roots showed higher antioxidant activity than control. In addition, transgenic hairy root harboring HMGR and DXR (HD42) exhibited higher tanshinone content after elicitation by yeast extract and/or Ag+ than before. Tanshinone can be significantly enhanced to 5.858, 6.716, and 4.426 mg g(-1) DW by YE, Ag+, and YE-Ag+ treatment compared with non-induced HD42, respectively. The content of cryptotanshinone and dihydrotanshinone was effectively elevated upon elicitor treatments, whereas there was no obvious promotion effect for the other two compounds tanshinone I and tanshinone IIA. Our results provide a useful strategy to improve tanshinone content as well as other natural active products by combination of genetic engineering with elicitors.
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