Journal
FUNCTIONAL & INTEGRATIVE GENOMICS
Volume 12, Issue 2, Pages 327-339Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-012-0271-6
Keywords
MicroRNA; Populus tomentosa; Drought; Flooding; High-throughput sequencing
Categories
Funding
- Forestry Public Benefit Research Program [201004009]
- Fundamental Research Funds for the Central Universities [YX2010-19]
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MicroRNAs (miRNAs) are a class of small, non-coding RNAs that play important downregulation roles in plants growth, development, and stress responses. To better identify Populus tomentosa miRNAs and understand the functions of miRNAs in response to water stress (drought and flooding), 152 conserved miRNAs belonging to 36 miRNA families, 8 known but non-conserved miRNAs and 64 candidate novel miRNAs belonging to 54 miRNA families were identified and analyzed from three small RNA (sRNA) libraries (drought treatment, flooding treatment, and control) by high-throughput sequencing combined with qRT-PCR. Significant changes in the expression of 17 conserved miRNA families and nine novel miRNAs were observed in response to drought stress, and in seven conserved miRNA families and five novel miRNAs in response to flooding stress. Both miRNA and miRNA*s were involved in the regulation of plant stress responses. The annotation of the potential targets of miRNAs with differential expression indicate that many types of genes encoding transcription factors, enzymes, and signal transduction components are implicated in the abiotic stress response..
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