Journal
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
Volume 78, Issue 18, Pages 1137-1141Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15287394.2015.1074970
Keywords
-
Funding
- University of Ottawa
Ask authors/readers for more resources
Gonad-mesonephros complexes from wood frog (Lithobates sylvaticus) tadpoles were incubated in vitro for 1 wk to examine direct effects of naphthenic acids (NA) and 17-ethinylestradiol (EE2) on ovarian differentiation. Histological images reveal successful preservation of gonadal integrity where cultured oocytes exhibited no significant differences in diameters and areas compared to uncultured conditions. Ovaries exposed to 10 g/L 17-EE2 contained oocytes with significantly advanced atresia and diminished areas and diameters, indicating a degree of ovarian regression. A significant reduction in oocyte area was observed in ovaries exposed to 3 mg/L of a commercial extract of petroleum-derived NA. This novel approach has applications for screening direct effects of endocrine-disrupting chemicals on gonadal function in tadpoles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available