Journal
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume 11, Issue 5, Pages 1562-1573Publisher
WILEY
DOI: 10.1002/term.2057
Keywords
alginate microspheres; basic fibroblast growth factor (bFGF); hydrogel; angiogenesis; burn; skin regeneration
Categories
Funding
- Natural Science Foundation of China [31271019, 51303064]
- Cooperative Research Project of the Chinese State Ministry of Education of Guangdong Province [2009B090300155]
- Scientific and Technological Project of Guangdong Province [2011B030100006]
- Scientific and Technological Project of Guangzhou [10A31020039, 11A22070526]
- Scientific and Technological Project of Guangzhou Tianhe District [104ZH084]
- Scientific and Technological Innovation Project of Guangdong Province Higher School [cxzd1109]
- Major State Basic Research Programme of China [2011CB8910701]
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Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis. Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing. Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity. To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)-poly(vinyl alcohol) (PVA) to form a composite hydrogel. Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS-PVA. In an in vitro study, the release of bFGF from Ms-CMCS-PVA in a sustained manner retained higher bioactivity over a 2-week period. Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS-PVA hydrogel, with and without bFGF. Compared with the control, CMCS-PVA and bFGF-CMCS-PVA groups, the bFGF/Ms-CMCS-PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis. Moreover, the bFGF/Ms-CMCS-PVA group had the highest density of newly formed and mature blood vessels during the 2 m week treatment period. The ability of the bFGF/Ms-CMCS-PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration. Copyright (C) 2015 John Wiley & Sons, Ltd.
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