Journal
FUEL
Volume 112, Issue -, Pages 331-337Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.fuel.2013.05.064
Keywords
Ethanol yield; Box-Behnken design (BBD); Response surface methodology (RSM); Simultaneous saccharification and fermentation (SSF); Wheat straw (WS)
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Funding
- Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07101-015-003]
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With the goal of obtaining a high ethanol yield, wheat straw (WS) was pretreated with a 1.0% (w/w) NaOH solution. Further parameters, including temperature, enzyme loading, yeast concentration and pH that would affect the ethanol yield through simultaneous saccharification and fermentation (SSF), were optimised by means of Box-Behnken design (BBD), a response surface methodology (RSM). All of the experiments were carried out with an initial solid content of 16.0% (w/v) and fermentation time of 120 h. A well fitted regression equation with an R-2 value of 0.9603 was obtained. The predicted maximum response value (ethanol yield) was 69.49% under the optimised conditions for SSF: temperature of 37.5 degrees C, enzyme loading of 30 FPU/g substrate, yeast concentration of 10 g/L and pH of 4.6. The validation experimental result showed that the maximum ethanol yield was 70.76%, which was in accordance with the predicted value. (C) 2013 Elsevier Ltd. All rights reserved.
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