Journal
FUEL
Volume 92, Issue 1, Pages 89-93Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.fuel.2011.06.048
Keywords
Pistacia chinensis bge; Rhizopus oryzae; Biodiesel; Resin; Transesterification
Categories
Funding
- National Natural Science Foundation of China [30901138]
- State Forestry Administration of China [2009-4-63]
- National High-Tech Research and Development Program of China 401 [2009AA05Z437]
- National Industry Special Project of China [201004001]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Biodiesel fuel from renewable non-edible woody plant oils has recently attracted more attention due to its environmental benefits and the reduced costs of raw materials. This study investigated the enzymatic transesterification of Pistacia chinensis bge seed oil (PCO) with methanol. The recombinant Rhizopus oryzae lipases (ROL) immobilized on macroporous resin and anion exchange resin, named as MI-ROL and AI-ROL, respectively, were used as biocatalysts. The transesterification reaction catalyzed by the immobilized lipase was investigated in a solvent-free system. The highest biodiesel yields of 92% and 94% were achieved under the optimum conditions (enzyme dosage 25 IUAI-ROL/g PCO or 7 IUMI-ROL/g PCO, methanol to oil molar ratio 5:1, water content 20% by weight of oil, temperature 37 degrees C, and reaction time 60 h). There was no obvious loss in the yield of biodiesel after being consecutively used for five cycles in the transesterification reactions using AI-ROL, while the yield of biodiesel remained above 60% after the MI-ROL was repeatedly used for four cycles. (C) 2011 Elsevier Ltd. All rights reserved.
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