Journal
FREE RADICAL RESEARCH
Volume 46, Issue 5, Pages 637-644Publisher
INFORMA HEALTHCARE
DOI: 10.3109/10715762.2012.664841
Keywords
extracellular-superoxide dismutase; protein kinase C; mitogen-activated protein kinase; reactive oxygen species; monocytic differentiation
Categories
Funding
- Japan Society for the Promotion for Science [23790190, 21590169]
- Grants-in-Aid for Scientific Research [23790190, 21590169] Funding Source: KAKEN
Ask authors/readers for more resources
Extracellular-superoxide dismutase (EC-SOD) is a major SOD isozyme mainly present in the vascular wall. EC-SOD is also observed in monocytes/macrophages, and its high expression contributes to the suppression of atherosclerosis by scavenging superoxide. The molecular mechanisms governing cell-specific expression of EC-SOD are mostly unknown, while the anti-oxidative effect of EC-SOD is well recognized. In this study, we investigated the expression of EC-SOD during 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced monocytic differentiation of THP-1 cells, which is not expressing its gene in the basal phase. We confirmed the significant induction of EC-SOD in a TPA time-dependent manner, and that induction was completely blocked by pre-treatment with GF109203X, an inhibitor of protein kinase C, U0126 and PD98059, inhibitors of mitogen-activated protein kinase kinase/extracellular-signal regulated kinase. Moreover, we determined the involvement of NADPH oxidase-derived reactive oxygen species in that induction. Overall, we considered that these results may contribute to clarify the cell-specific expression of EC-SOD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available