Journal
FREE RADICAL RESEARCH
Volume 44, Issue 8, Pages 854-863Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10715762.2010.485995
Keywords
Ischemia-reperfusion injury; n-3 polyunsaturated fatty acid; liver preconditioning; oxidative stress; pro-inflammatory cytokines; NF-kappa B activation
Categories
Funding
- FONDECYT, Chile [1080039]
Ask authors/readers for more resources
N-3 polyunsaturated fatty acids (n-3 PUPA) affect inflammatory processes. This study evaluated the effects of dietary supplementation with fish oil on hepatic ischemia-reperfusion (IR) injury in the rat. Parameters of liver injury (serum transaminases and histology) and oxidative stress (serum 8-isoprostanes and hepatic GSH and GSSG), were correlated with NF-kappa B DNA binding and FA composition and inflammatory cytokine release. N-3 PUFA supplementation significantly increased liver n-3 PUFA content and decreased n-6/n-3 PUFA ratios. IR significantly modified liver histology and enhanced serum transaminases, 8-isoprotanes and inflammatory cytokines, with net reduction in liver GSH levels and net increment in those of GSSG. Early increase (3 h) and late reduction (20 h) in NF-kappa B activity was induced. All IR-induced changes were normalized by n-3 PUFA supplementation. In conclusion, prevention of liver IR-injury was achieved by n-3 PUPA supplementation, with suppression of oxidative stress and recovery of pro-inflammatory cytokine homeostasis and NF-kappa B functionality lost during IR.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available