Journal
FREE RADICAL RESEARCH
Volume 45, Issue 4, Pages 439-444Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10715762.2010.540575
Keywords
Cytochrome c; peroxide; ascorbate
Categories
Funding
- Romanian Ministry of Education and Research [PCCE 140//2008]
- [POSDRU/88/1.5/S/60185]
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The peroxidase-type reactivity of cytochrome c is proposed to play a role in free radical production and/or apoptosis. This study describes cytochrome c catalysis of peroxide consumption by ascorbate. Under conditions where the sixth coordination position at the cytochrome c heme iron becomes more accessible for exogenous ligands (by carboxymethylation, cardiolipin addition or by partial denaturation with guanidinium hydrochloride) this peroxidase activity is enhanced. A reaction intermediate is detected by stopped-flow UV-vis spectroscopy upon reaction of guanidine-treated cytochrome c with peroxide, which resembles the spectrum of globin Compound II species and is thus proposed to be a ferryl species. The ability of physiological levels of ascorbate (10-60 mu M) to interact with this species may have implications for mechanisms of cell signalling or damage that are based on cytochrome c/peroxide interactions.
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