4.3 Article

Thermal generation of stable [image omitted] spin trap adducts with super-hyperfine structure in their EPR spectra: An alternative EPR spin trapping assay for radical scavenging capacity determination in dimethylsulphoxide

Journal

FREE RADICAL RESEARCH
Volume 43, Issue 5, Pages 457-U19

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760902846140

Keywords

EPR spectroscopy; spin trapping; DMSO; sulphate radical anion; antioxidant

Funding

  1. Research and Development Agency of the Slovak Republic [APVT-20-004504, APVV-0310-06]
  2. Scientific Grant Agency of the Slovak Republic [VEGA/1/3579/06, 1/0018/09, 1/0845/08]

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Thermal decomposition of potassium persulphate (K2S2O8) was studied in detail by the EPR spin trapping technique in dimethylsulphoxide (DMSO), employing 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), 5-ethoxycarbonyl-5-methyl-1-pyrroline-N-oxide (EMPO) and 5-(diisopropoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DIPPMPO) as spin traps. DMPO and/or its derivatives exclusively trapped the primary formed SO4 - radical anions producing stable spin adducts with half-lives exceeding 2 h at room temperature. High-resolution EPR spectra of these adducts showed unusually rich hyperfine structure due to the interaction of the unpaired electron with all magnetically active nuclei of the spin trap moiety. In contrast to aprotic DMSO solvent, DMPO-OH adducts dominated in mixed DMSO/water solutions with water content higher than 50%. The thermal decomposition of K2S2O8 in DMSO represents an effective source of free radicals for the radical scavenging capacity (RSC) determination assay, applicable to hydrophilic as well as hydrophobic antioxidants. Efficiency of the assay is demonstrated with a series of cereal samples.

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