Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 65, Issue -, Pages 719-723Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2013.07.044
Keywords
Sperm nuclear organization; Paternal DNA integrity; Free radicals
Funding
- French Ministry of Higher Education and Research (MESR)
- INSERM
- CNRS
- MESR
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Gamete DNA integrity is one key parameter conditioning reproductive success as well as the quality of life for the offspring. In particular, damage to the male nucleus can have profound negative effects on the outcome of fertilization. Because of the absence of repair activity of the quiescent mature spermatozoa it is easily subjected to nuclear damage, of which oxidative damage is by far the most prominent. In relation to the organization of the mammalian sperm nucleus we show here that one can correlate the nuclear regions of lower compaction with areas preferentially showing oxidative damage. More precisely, we show that oxidative DNA damage targets primarily histone-rich and nuclear matrix-attached domains located in the peripheral and basal regions of the mouse sperm nucleus. These particular sperm DNA domains were recently shown to be enriched in genes of paramount importance in postfertilization DNA replication events and in the onset of the embryonic developmental program. We propose that monitoring of sperm DNA oxidation using the type of assay presented here should be considered in clinical practice when one wants to estimate the integrity of the paternal nucleus along with more classical assays that essentially analyze DNA fragmentation and nucleus compaction. (C) 2013 Elsevier Inc. All rights reserved.
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