Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 52, Issue 3, Pages 626-634Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2011.11.019
Keywords
Oxidative stress; Dual role of melanin; Photoprotection; Photosensitizer; Pigment; DHR-123; MitoSOX; Mitochondria; Ultraviolet radiation; Human skin; Free radicals
Funding
- Cancer Research UK [C1474/A10235]
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Skin cancer incidence is dramatically increasing worldwide, with exposure to ultraviolet radiation (UVR) a predominant factor. The UVA component initiates oxidative stress in human skin, although its exact role in the initiation of skin cancer, particularly malignant melanoma, remains unclear and is controversial because there is evidence for a melanin-dependent mechanism in UVA-linked melanoma studies. Nonpigmented (CHL-1, A375), moderately pigmented (FM55, SKme123), and highly pigmented (FM94, hyperpigmented FM55) human melanoma cell lines have been used to investigate UVA-induced production of reactive oxygen species using FACS analysis, at both the cellular (dihydrorhodamine-123) and the mitochondria! (MitoSOX) level, where most cellular stress is generated. For the first time, downstream mtDNA damage (utilizing a quantitative long-PCR assay) has been investigated. Using UVA, UVB, and H2O2 as cellular stressors, we have explored the dual roles of melanin as a photoprotector and photosensitizer. The presence of melanin has no influence over cellular oxidative stress generation, whereas, in contrast, melanin protects against mitochondrial superoxide generation and mtDNA damage (one-way ANOVA with post hoc Tukey's analysis, P<0.001). We show that if melanin binds directly to DNA, it acts as a direct photosensitizer of mtDNA damage during UVA irradiation (P<0.001), providing evidence for the dual roles of melanin. (C) 2011 Elsevier Inc. All rights reserved.
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