Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 52, Issue 11-12, Pages 2246-2253Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2012.04.001
Keywords
Respiratory burst; PUFA; Macrophages; Reactive nitrogen intermediates; Reactive oxygen intermediates; Rhodococcus equi; Pseudomonas aeruginosa; Free radicals
Funding
- Deutsche Forschungsgemeinschaft [SCHU 2586/1-1]
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In this paper, using the monocyte/macrophage cell line RAW264.7, we systematically investigate the impact of macrophage enrichment with unsaturated fatty acids on cellular radical synthesis. We found that the intracellular production of reactive nitrogen and oxygen intermediates depends on the activation status of the macrophages. For unstimulated macrophages PUFA enrichment resulted in an increase in cellular radical synthesis. For stimulated macrophages, instead, an impeding action of unsaturated fatty acids on the respiratory burst could be seen. Of particular importance, the impact of unsaturated fatty acids on the macrophage respiratory burst was also observed in RAW264.7 cells cocultivated with viable bacteria of the species Rhodococcus equi or Pseudomonas aeruginosa. PUFA supplementation of macrophages in the presence of R. equi or P. aeruginosa reduced the pathogen-stimulated synthesis of reactive nitrogen and oxygen intermediates. Furthermore, the unsaturated fatty acids were found to impede the expression of the myeloperoxidase gene and to reduce the activity of the enzyme. Hence, our data provide indications of a possible value of PUFA application to people suffering from chronic infections with R. equi and P. aeruginosa as a concomitant treatment to attenuate an excessive respiratory burst. (C) 2012 Elsevier Inc. All rights reserved.
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