Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 52, Issue 9, Pages 1750-1759Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2012.02.027
Keywords
Oxidative stress; Antioxidants; Cyanidin 3-glucoside; Vascular endothelium; Bilitranslocase
Funding
- Slovenian Research Agency [J3-0024]
- Slovene Human Resources Development and Scholarship Fund (Ad Futura Grant for International Mobility)
- Autonomous Province of Trento (ADP)
- Autonomous Province of Trento (METAQUALITY)
- Fondazione Cassa di Risparmio di Trieste
- European Regional Development Fund, Cross-Border Cooperation Italy-Slovenia Programme (Progetto Strategico TRANS2CARE)
Ask authors/readers for more resources
Flavonoids are dietary components involved in decreasing oxidative stress in the vascular endothelium and thus the risk of endothelial dysfunction. However, their very low concentrations in plasma place this role in doubt. Thus, a relationship between the effective intracellular concentration of flavonoids and their bioactivity needs to be assessed. This study examined the uptake of physiological concentrations of cyanidin 3-glucoside, a widespread dietary flavonoid, into human vascular endothelial cells. Furthermore, the involvement of the membrane transporter bilitranslocase (TC No. 2.A.65.1.1) as the key underlying molecular mechanism for membrane transport was investigated by using purified anti-sequence antibodies binding at the extracellular domain of the protein. The experimental observations were carried out in isolated plasma membrane vesicles and intact endothelial cells from human endothelial cells (EA.hy926) and on an ischemia-reperfusion model in isolated rat hearts. Cyanidin 3-glucoside was transported via bilitranslocase into endothelial cells, where it acted as a powerful intracellular antioxidant and a cardioprotective agent in the reperfusion phase after ischemia. These findings suggest that dietary flavonoids, despite their limited oral bioavailability and very low post-absorption plasma concentrations, may provide protection against oxidative stress-based cardiovascular diseases. Bilitranslocase, by mediating the cellular uptake of some flavonoids, is thus a key factor in their protective activity on endothelial function. (c) 2012 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available