Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 52, Issue 1, Pages 1-6Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2011.09.030
Keywords
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Funding
- British Heart Foundation [FS/10/59/28533] Funding Source: Medline
- Medical Research Council [G0800971] Funding Source: Medline
- NHLBI NIH HHS [R01 HL058752, R01 HL063119] Funding Source: Medline
- NIAAA NIH HHS [R01 AA013395] Funding Source: Medline
- NIEHS NIH HHS [R56 ES003598, R01 ES005511, R21 ES020942, P30 ES013508, R01 ES003598] Funding Source: Medline
- MRC [G0800971] Funding Source: UKRI
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL063119, R01HL058752] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES005511, R56ES003598, R21ES020942, P30ES013508, R01ES003598] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM [R01AA013395] Funding Source: NIH RePORTER
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The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.
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