Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 52, Issue 1, Pages 80-87Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2011.09.024
Keywords
nitric oxide; cytochrome c; nitrogen dioxide; free radical; urate; mitochondria
Funding
- UK Biological and Biotechnological Sciences Research Council [BB/D017858/1]
- Wellcome Trust [69769]
- Biochemical Society Summer Studentshi
- Biotechnology and Biological Sciences Research Council [BB/D017858/1] Funding Source: researchfish
- BBSRC [BB/D017858/1] Funding Source: UKRI
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We previously reported that high micromolar concentrations of nitric oxide were able to oxidize mitochondrial cytochrome c at physiological pH, producing nitroxyl anion (Sharpe and Cooper, 1998 Biochem. J. 332, 9-19). However, the subsequent re-evaluation of the redox potential of the NO/NO(-) couple suggests that this reaction is thermodynamically unfavored. We now show that the oxidation is oxygen-concentration dependent and non stoichiometric. We conclude that the effect is due to an oxidant species produced during the aerobic decay of nitric oxide to nitrite and nitrate. The species is most probably nitrogen dioxide, NO a well-known biologically active oxidant. A simple kinetic model of NO autoxidation is able to explain the extent of cytochrome c oxidation assuming a rate constant of 3 x 10(6) M(-1) s(-1) for the reaction of NCO(2)(center dot) with ferrocytochrome c. The importance of NO(2)(center dot) was confirmed by the addition of scavengers such as urate and ferrocyanide. These convert NO into products (urate radical and ferricyanide) that rapidly oxidize cytochrome c and hence greatly enhance the extent of oxidation observed. The present study does not support the previous hypothesis that NO and cytochrome c can generate appreciable amounts of nitroxyl ions (NO or HNO) or of peroxynitrite. (C) 2011 Elsevier Inc. All rights reserved.
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