4.7 Article

HIV-1 Tat increases oxidant burden in the lungs of transgenic mice

Journal

FREE RADICAL BIOLOGY AND MEDICINE
Volume 51, Issue 9, Pages 1697-1707

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2011.07.023

Keywords

HIV; Tat; Oxidative stress; NF-kappa B; Inflammation; TxNIP; Free radicals

Funding

  1. National Heart, Lung, and Blood Institute [5R01HL059785]

Ask authors/readers for more resources

Chronic human immunodeficiency virus infection is associated with higher incidence of pulmonary complications including hypertension, vasculopathy, lymphocytic alveolitis, and interstitial pneumonitis not attributed to either opportunistic infections or presence of the virus. The Tat (transactivator of transcription) protein, a required transactivator for expression of full-length viral genes, is pleiotropic and influences expression of cellular inflammatory genes. Tat-dependent transactivation of cellular genes requires specific mediators, including NF-kappa B, widely recognized as sensitive to changes in cellular oxidant burden. We hypothesized that overproduction of Tat leads to increased oxidant burden and to alterations in basal inflammatory status as measured by NF-kappa B activation. We engineered transgenic mouse lines that express Tat (86-amino-acid isoform) in the lung under the control of the surfactant protein C promoter. Tat-transgenic mice exhibit increased pulmonary cellular infiltration, increased nitrotyrosine and carbonyl protein modifications, and increased levels of NF-kappa B, MnSOD, and thioredoxin-interacting protein. These data indicate that Tat increases oxidant burden and resets the threshold for inflammation, which may increase susceptibility to secondary injuries. Published by Elsevier Inc.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available