Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 48, Issue 9, Pages 1173-1181Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2010.01.036
Keywords
Radical scavenging peptide; Amphibian skin; Redox homeostasis; Free radicals
Funding
- Chinese National Natural Science Foundation [30830021]
- Chinese Academy of Sciences [KSCX2-YW-G-024]
- Ministry of Science [2008AA02Z133, 2010CB529801/05]
- Ministry of Science Yunnan Province [0803461101]
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The question of how amphibians can protect themselves from reactive oxygen species when exposed to the sun in an oxygen-rich atmosphere is important and interesting, not only from an evolutionary viewpoint, but also as a primer for researchers interested in mammalian skin biology, in which such peptide systems for antioxidant defense are not well studied. The identification of an antioxidant peptide named antioxidin-RL from frog (Odorrana livida) skin in this report supports the idea that a peptide antioxidant system may be a widespread antioxidant strategy among amphibian skins. Its ability to eliminate most of the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical tested within 2 s, which is much faster than the commercial antioxidant factor butylated hydroxytoluene, suggests that it has a potentially large impact on redox homeostasis in amphibian skins. Cys10 is proven to be responsible for its rapid radical scavenging function and tyrosines take part in the binding of antioxidin-RL to radicals according to our nuclear magnetic resonance assay. (C) 2010 Elsevier Inc. All rights reserved.
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