Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 44, Issue 6, Pages 1023-1031Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2007.11.021
Keywords
TRP-2; dopachrome tautomerase; melanocytes; oxidative stress; RNAi; comet assay; GSH
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TRP-2 (dopachrome tautomerase) is a melanogenic enzyme whose expression was recently reported to modulate melanocyte response to different cytotoxic events. Here we studied a possible role of TRP-2 in the oxidative stress response in the amelanotic WM35 melanoma cell line. Cell viability assays showed that TRP-2 overexpression in WM35 cells reduced their sensitivity to oxidative stress. Comet assays linked TRP-2 expression to DNA damage protection, and high-performance liquid chromotography-tandem mass spectrometry experiments showed an increase in intracellular glutathione in TRP-2-overexpressing cells. These effects were specifically reversed when TRP-2 was silenced by RNA interference. Nevertheless, these properties appeared to depend on a particular cell environment because expression of TRP-2 failed to rescue HEK epithelial cells exposed to similar treatments. (c) 2007 Elsevier Inc. All rights reserved.
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