Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 44, Issue 11, Pages 1926-1934Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2008.02.011
Keywords
diffusion; fibroblasts; kinetics; near infrared; photodynamic therapy; photosensitizer; porphyrin; singlet oxygen; subcellular localization; time resolved
Ask authors/readers for more resources
The roles played by singlet oxygen (O-1(2)) in photodynamic therapy are not fully understood yet. In particular, the mobility of O-1(2) within cells has been a subject of debate for the last two decades. In this work, we report on the kinetics of O-1(2) formation, diffusion, and decay in human skin fibroblasts. O-1(2) has been photosensitized by two water-soluble porphyrins targeting different subcellular organelles, namely the nucleus and lysosomes, respectively. By recording the time-resolved near-IR phosphorescence of O-1(2) and that of its precursor the photosensitizer's triplet state, we find that the kinetics of singlet oxygen formation and decay are strongly dependent on the site of generation. O-1(2) photosensitized in the nucleus is able to escape out of the cells while O-1(2) photosensitized in the lysosomes is not. Despite showing a lifetime in the microsecond time domain, O-1(2) decay is largely governed by interactions with the biomolecules within the organelle where it is produced. This observation may reconcile earlier views that singlet oxygen-induced photodamage is highly localized, while its lifetime is long enough to diffuse over long distances within the cells. (C) 2008 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available