Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 44, Issue 10, Pages 1857-1862Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2008.02.003
Keywords
oxygen; anaerobe; Prevotella melaninogenica; 8-oxo-deoxyguanosine; mutation; rpoB; rifampicin; free radicals
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Strict anaerobes are highly sensitive to oxygen, but the mutagenicity of oxygen in strict anaerobes has not been well understood. Prevotella melaninogenica, a strict anaerobe, is susceptible to oxygen and shows an increase in oxidative DNA damage upon exposure to oxygen. In this study, we have investigated the mutagenicity of oxygen and the types of mutations induced by oxygen. Exposure to oxygen decreased cell survival and increased the levels of 8-oxo-deoxyguanosine (8-oxodG). The frequency of rifampicin-resistant mutants was markedly increased after exposure to oxygen. After sequencing a 254-bp fragment of the rpoB gene, which encodes the 13 subunit of bacterial RNA polymerase, a target molecule of rifampicin, we found that most mutants induced by oxygen had GC to TA transversions, a signature of 8-oxodG. In addition, all detected single-nucleotide changes would lead to amino acid changes that confer rifampicin resistance. These results indicate that oxygen is mutagenic in a strict anaerobe, P. melaninogenica, and its mutagenic characteristics could be analyzed with this experimental system. (C) 2008 Elsevier Inc. All rights reserved.
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