Journal
FORESTRY
Volume 82, Issue 2, Pages 175-184Publisher
OXFORD UNIV PRESS
DOI: 10.1093/forestry/cpn052
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Funding
- Ministerio de Educacion y Ciencia de Espana [AP2005-0140]
- Ministerio de Ciencia y Tecnologia de Espana [CN-05-169-IE03-091, AGL2005-08214-C02-01/FOR, AGL2006-07360/FOR]
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In this work an improved micropropagation protocol for Pinus pinaster Ait. using isolated cotyledons is presented. The variables studied during bud induction were embryo preculture, type and concentration of cytokinin and influence of illumination culture conditions. Shoot development and proliferation was obtained by transfer to hormone-free medium with activated charcoal. This protocol also describes a procedure for successful continuous multiplication using axillary shoots and brachyblasts formed during shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be expected per seed after 16 weeks.
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