Journal
FORENSIC SCIENCE INTERNATIONAL
Volume 231, Issue 1-3, Pages 157-166Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.forsciint.2013.04.033
Keywords
Raman spectroscopy; Blood detection; Substrate interference; Forensic science; Statistical analysis
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Funding
- National Institute of Justice, Office of Justice Programs, U.S. Department of Justice
- [2011-DN-BX-K551]
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Raman spectroscopy has demonstrated remarkable capabilities in identifying blood in controlled laboratory conditions. However, substrate interference presents a significant challenge toward characterizing body fluid traces with Raman spectroscopy at a crime scene. Here, several possible solutions are explored, including the selection of laser excitation, isolating the signal of blood using spectral subtraction and using a favorable substrate for collection which minimizes interference. Simulated blood stain evidence was prepared and analyzed using a Raman microscope with variable laser capabilities. It is shown that the best approach for detecting blood depends on the nature of the substrate and the type of interference encountered. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
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