Journal
FORENSIC SCIENCE INTERNATIONAL
Volume 184, Issue 1-3, Pages 64-68Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.forsciint.2008.12.002
Keywords
MDMA; Metabolites; LC-ESI-MS; Human plasma; Validation; Acidic hydrolysis
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Funding
- USPHS [DA05707]
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3,4-Methylenedioxymethamphetamine (MDMA, Ecstasy) is a psychoactive drug with abuse liability and neurotoxic potential. Specimen preparation of a recently presented LC-MS assay with electrospray ionization for quantifying MDMA and its main metabolites in squirrel monkey plasma was modified to include acidic hydrolysis to obtain total 3,4-dihydroxymethamphetamine and 4-hydroxy-3-methoxymethamphetamine. Method re-validation for squirrel monkey plasma and full validation for human plasma showed selectivity for all analytes. Recoveries were >= 71.0%. Changed specimen preparation or matrix did not affect accuracy or precision. No instability was observed after repeated freezing or in processed samples. Plasma MDMA and metabolites quantification, derived pharmacokinetic and toxicokinetic data and neurotoxicity research will benefit from this validated method. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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