Journal
FOODBORNE PATHOGENS AND DISEASE
Volume 11, Issue 9, Pages 734-739Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/fpd.2014.1749
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Funding
- National Basic Research Program of China (973 Program) [2012CB722501]
- Special Fund for Agro-scientific Research in the Public Interest [201303042]
- Chinese National Nature Science Foundation [31372430]
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The precise detection of Toxoplasma gondii infection in cattle has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for T. gondii infection in cattle by an indirect enzyme-linked immunosorbent assay (ELISA) using the reference serum samples determined by immunofluorescence assay and Western blotting, showing that GRA7-ELISA has a sensitivity of 96.4%, and a specificity of 98.6%. The detection results by GRA7-and Toxoplasma lysate antigen-based ELISAs were compared, indicating that no significant difference (p > 0.05) and perfect agreement (k = 0.74) was observed between the two tests. Receiver operating characteristic analysis showed a relative sensitivity and specificity of 100% and 97.3% at the cut-off value of 0.3 for GRA7-ELISA. These data demonstrate that GRA7 is a promising serodiagnostic marker for T. gondii infection in cattle.
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