Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 21, Issue 1, Pages 101-106Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-012-0012-6
Keywords
Listeria monocytogenes; loop-mediated isothermal amplication (LAMP); real-time turbidimeter; quantitative
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Funding
- National Natural Science Foundation of China [20877028]
- Major State Basic Research Development Program of China (973 Program) [2010CB735700]
- Guangdong Nature Science Foundation of China [10451064101005159]
- SCUT [2009ZM0224]
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The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63 degrees C with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6 colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional method-positive samples was missed by the LAMP method.
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