Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 20, Issue 2, Pages 445-454Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-011-0062-1
Keywords
Rhodotorula acheniorum; whey ultrafiltrate; beta-carotene; mutagenesis; response surface methodology
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Funding
- Council for Research at the Campus of Agriculture and Natural Research
- Research Council of the University of Tehran
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The present work investigated on carotenogenesis with high beta-carotene content by a new isolated high-activity strain-producer Rhodotorula acheniorum mutant MRN in cheese whey ultrafiltrate. After a serial of UV, ethymethanesurfonate (EMS), and nitrosoguanidine (NTG) mutagenesis, a mutant named MRN of the red lactose-positive yeast strain R. acheniorum was obtained. Then, the effects of different growth medium factors on carotenoid production by this mutant at batch-scale level were identified and optimized by means of response surface methodology (RSM) in order to achieve high-level production of beta-carotene. The optimum conditions required to achieve the highest level of beta-carotene (262.12 +/- 1.01 mg/L) were determined as follows: whey ultrafiltrate (WU) lactose concentration 55 g/L, pH 5.85, ammonium sulfate concentration 3.5 g/L, temperature 23A degrees C, and aeration rate 1.56 vvm. The medium optimization resulted in a 6.45-fold increase in volumetric production (262.12 +/- 1.01 mg/L) and a 4.62-fold increase in the cellular accumulation (10.69 +/- 0.19 mg/g) of beta-carotene.
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