Journal
FOOD RESEARCH INTERNATIONAL
Volume 116, Issue -, Pages 660-667Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2018.08.093
Keywords
Lutein; Carotenoids; Microalgae; High throughput screening; Marine; 18S rRNA genes sequencing analysis; Phylogeny; HPLC-DAD-MS
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Funding
- Asahi Kasei Corporation
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A novel highly selective lutein-producing marine microalga was isolated and identified using high throughput screening (HTS). Phylogenetic analysis based on 18S rRNA gene sequence revealed it belongs to a microalgae class (Trebouxiophyceae), and is closely related to Auxenochlorella spp. The novel strain was designated as Auxenochlorella sp. strain LEU27. Based on HPLC-DAD and HPLC-MS analyses, strain LEU27 produced a total of 1203.9 +/- 98 mu g g(-1) dry cells of carotenoids, including a remarkable amount (996.6 +/- 98 mu g g(-1) dry cells) of pure lutein under heterotrophic growth conditions. This is the first report that describes a natural, highly selective lutein-producing marine microalga isolated by HTS approach.
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