Journal
FOOD RESEARCH INTERNATIONAL
Volume 116, Issue -, Pages 518-526Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2018.08.068
Keywords
Walnut meal protein; Antioxidant peptides; Gastrointestinal digestion; Radical scavenging activity; Cell viability
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Funding
- National Natural Science Foundation of China [31501467]
- Shaanxi Province Key Research and Development Plan [2017NY-157]
- Shaanxi Science & Technology Co-ordination & Innovation Project [2016KTCQ02-22]
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The aim of this study was to isolate and identify antioxidant peptides from defatted walnut meal proteins hydrolysates (DWMPH) prepared by simulated gastrointestinal digestion, and to evaluate the protective effect of the selected antioxidant peptides against oxidative damage to SH-SY5Y cells. The DWMPH were purified by ultrafiltration, gel filtration chromatography and RP-HPLC successively, and finally six major peptides were identified as VRN, NPAN, AHSVGP, SSE, TY and SGGY by UPLC-QTOF-MS. TY and SGGY displayed stronger ABTS radical scavenging activity and higher ORAC value in comparison with other four peptides. Furthermore, 0.5 mg/mL SGGY exhibited significantly protective effect on SH-SY5Y cells against oxidative damage (p < .01). Therefore, it was suggested that walnut meal protein could serve as a potential source of antioxidative peptides through gastrointestinal digestion.
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